Sebastian Barg
Professor at Institutionen för medicinsk cellbiologi; Forskargrupp Sebastian Barg
- Telephone:
- +46 18 471 46 60
- E-mail:
- sebastian.barg@mcb.uu.se
- Visiting address:
- BMC
Husargatan 3
751 23 Uppsala - Postal address:
- Box 571
751 23 UPPSALA
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Short presentation
My research group studies the cell biology of insulin secretion using advanced light microscopy (TIRF, single molecule imaging) and patch clamp electrophysiology; a particular focus is on the regulated exocytosis of secretory granules. I teach cell biology and general endocrinology.
Biography
- 1995 MSc Biochemistry Free University Berlin
- 1995 undergraduate thesis Vanderbilt University (with Sid Fleischer)
- 2001 PhD Medical Sciences Lund University (with P Rorsman)
- 2002 Assistant professor (Swedish Science Council/Vetenskapsrådet)
- 2002-06 postdoc Vollum Institute Portland OR (with W Almers)
- 2007 research assistant professor Oregon Health & Science University
- 2007 lecturer Imperial College London
- 2008 Assistant professor Uppsala University
- 2011 Associate professor Uppsala University
honors:
- 2001 best PhD thesis Medical faculty Lund
- 2002 Swedish Research Council (VR) assistant professor position
- 2002 EMBO longterm fellowship
- 2002 STINT fellowship award
- 2002 Swedish Society for Medical Research fellowship award
- 2002 Albert Renold Career Development Award (EFSD)
- 2005 Wallenberg fellowship award in Nano/micro Science
- 2008 Zetterling fellowship award
- 2008-11 Göran Gustafsson award in human biology
Publications
Selection of publications
- Paracrine control of α-cell glucagon exocytosis is compromised in human type-2 diabetes. (2020)
- Fusion pore regulation by cAMP/Epac2 controls cargo release during insulin exocytosis (2019)
- Glucose-Dependent Granule Docking Limits Insulin Secretion and Is Decreased in Human Type 2 Diabetes (2018)
- Syntaxin clusters at secretory granules in a munc18-bound conformation (2018)
- Ca2+ channel clustering with insulin-containing granules is disturbed in type 2 diabetes (2017)
- Contact-induced clustering of syntaxin and munc18 docks secretory granules at the exocytosis site (2014)
- Syntaxin clusters assemble reversibly at sites of secretory granules in live cells (2010)
- Delay between fusion pore opening and peptide release from large dense-core vesicles in neuroendocrine cells (2002)
Recent publications
- SEC14-like condensate phase transitions at plasma membranes regulate root growth in Arabidopsis (2023)
- Local PI(4,5)P2 signaling inhibits fusion pore expansion during exocytosis (2023)
- The β-cell primary cilium is an autonomous Ca2+ compartment for paracrine GABA signaling (2023)
- Restoration of PITPNA in Type 2 diabetic human islets reverses pancreatic beta-cell dysfunction (2023)
- Functional, metabolic and transcriptional maturation of human pancreatic islets derived from stem cells. (2022)
All publications
Articles
- SEC14-like condensate phase transitions at plasma membranes regulate root growth in Arabidopsis (2023)
- Local PI(4,5)P2 signaling inhibits fusion pore expansion during exocytosis (2023)
- The β-cell primary cilium is an autonomous Ca2+ compartment for paracrine GABA signaling (2023)
- Restoration of PITPNA in Type 2 diabetic human islets reverses pancreatic beta-cell dysfunction (2023)
- Functional, metabolic and transcriptional maturation of human pancreatic islets derived from stem cells. (2022)
- Alternative splicing encodes functional intracellular CD59 isoforms that mediate insulin secretion and are down-regulated in diabetic islets (2022)
- Paracrine control of α-cell glucagon exocytosis is compromised in human type-2 diabetes. (2020)
- In pancreatic islets from type 2 diabetes patients, the dampened circadian oscillators lead to reduced insulin and glucagon exocytosis (2020)
- Fusion pore regulation by cAMP/Epac2 controls cargo release during insulin exocytosis (2019)
- The Endoplasmic Reticulum Chaperone Glucose-Regulated Protein 94 is Essential for Proinsulin Handling (2019)
- Glucose-Dependent Granule Docking Limits Insulin Secretion and Is Decreased in Human Type 2 Diabetes (2018)
- Functional Characterization of Native, High-Affinity GABAA Receptors in Human Pancreatic β Cells (2018)
- PtdIns(4,5)P2 is not required for secretory granule docking (2018)
- Syntaxin clusters at secretory granules in a munc18-bound conformation (2018)
- Recruitment of Epac2A to Insulin Granule Docking Sites Regulates Priming for Exocytosis (2017)
- Ca2+ channel clustering with insulin-containing granules is disturbed in type 2 diabetes (2017)
- Plasma membrane PI(4,5)P-2 is critical for secretory granule exocytosis (2017)
- MiR-335 overexpression impairs insulin secretion through defective priming of insulin vesicles (2017)
- How Kiss-and-Run Can Make Us Sick (2016)
- Statistical Frailty Modeling for Quantitative Analysis of Exocytotic Events Recorded by Live Cell Imaging (2016)
- Plasma Membrane Phosphatidylinositol 4,5-Bisphosphate Regulates Ca2+-Influx and Insulin Secretion from Pancreatic beta Cells (2016)
- Quantitative Imaging of the Exocytosis Machinery Assembly (2015)
- Survey of Red Fluorescence Proteins as Markers for Secretory Granule Exocytosis (2015)
- G-protein coupled receptor dynamics in insulin secreting cells (2015)
- MiR-335 regulates exocytotic proteins and affects glucose-stimulated insulin secretion through decreased Ca2+-dependent exocytosis in beta cells (2015)
- Contact-induced clustering of syntaxin and munc18 docks secretory granules at the exocytosis site (2014)
- The Complement Inhibitor CD59 Regulates Insulin Secretion by Modulating Exocytotic Events (2014)
- CD59 is required for pancreatic beta cell insulin exocytosis (2014)
- In Intact Islets Interstitial GABA Activates GABA(A) Receptors That Generate Tonic Currents in alpha-Cells (2013)
- Aberrant association between vascular endothelial growth factor receptor-2 and VE-cadherin in response to vascular endothelial growth factor-a in Shb-deficient lung endothelial cells (2013)
- Multivesicular exocytosis in rat pancreatic beta cells (2012)
- PPARβ/δ affects pancreatic β cell mass and insulin secretion in mice (2012)
- Quantitative analysis of t-SNARE and Ca2+-channel clusters near secretory granules (2010)
- Syntaxin clusters assemble reversibly at sites of secretory granules in live cells (2010)
- cAMP Mediators of Pulsatile Insulin Secretion from Glucose-stimulated Single β-Cells (2010)
- Single secretory granules of live cells recruit syntaxin-1 and synaptosomal associated protein 25 (SNAP-25) in large copy numbers (2010)
- Defective secretion of islet hormones in chromogranin-B deficient mice (2010)
- TCF7L2 regulates late events in insulin secretion from pancreatic islet beta-cells (2009)
- High extracellular glucose inhibits exocytosis through disruption of syntaxin 1A-containing lipid rafts (2009)
- Impaired glucose homeostasis in Shb-/- mice (2009)
- Granule docking and cargo release in pancreatic β-cells (2008)
- Compensatory endocytosis in chromaffin cells (2008)
- Release of the styryl dyes from single synaptic vesicles in hippocampal neurons. (2008)
- Membrane phosphoinositides control insulin secretion through their effects on ATP-sensitive K+ channel activity. (2005)
- Selective nucleotide-release from dense-core granules in insulin-secreting cells (2005)
- Insulin secretion (2004)
- Large dense-core vesicle exocytosis in pancreatic beta-cells monitored by capacitance measurements. (2004)
- Mechanisms of exocytosis in insulin-secreting B-cells and glucagon-secreting A-cells. (2003)
- SUR1 regulates PKA-independent cAMP-induced granule priming in mouse pancreatic B-cells. (2003)
- A subset of 50 secretory granules in close contact with L-type Ca2+ channels accounts for first-phase insulin secretion in mouse beta-cells. (2002)
- Delay between fusion pore opening and peptide release from large dense-core vesicles in neuroendocrine cells (2002)
- Characterization of a naturally occurring mutation (L107I) in the HNF1 alpha (MODY3) gene. (2002)
- Differential activation by Ca2+, ATP and caffeine of cardiac and skeletal muscle ryanodine receptors after block by Mg2+. (2002)
- Effect of mutant alpha-synuclein on dopamine homeostasis in a new human mesencephalic cell line. (2002)
- Fast insulin secretion reflects exocytosis of docked granules in mouse pancreatic B-cells. (2002)
- Sulfonylurea-mediated stimulation of insulin exocytosis via an ATP-sensitive K+ channel-independent action. (2002)
- Priming of insulin granules for exocytosis by granular Cl(-) uptake and acidification (2001)
- Fast exocytosis with few Ca(2+) channels in insulin-secreting mouse pancreatic B cells (2001)
- Tight coupling between electrical activity and exocytosis in mouse glucagon-secreting alpha-cells. (2000)
- Patch-clamp characterisation of somatostatin-secreting -cells in intact mouse pancreatic islets. (2000)
- Regulation of glucagon release in mouse -cells by KATP channels and inactivation of TTX-sensitive Na+ channels. (2000)
- Tolbutamide stimulates exocytosis of glucagon by inhibition of a mitochondrial-like ATP-sensitive K+ (KATP) conductance in rat pancreatic A-cells. (2000)
- The Cell Physiology of Biphasic Insulin Secretion. (2000)
- The stimulatory action of tolbutamide on Ca2+-dependent exocytosis in pancreatic beta cells is mediated by a 65-kDa mdr-like P-glycoprotein (1999)
- Voltage-gated and resting membrane currents recorded from B-cells in intact mouse pancreatic islets. (1999)
- Activation of Ca(2+)-dependent K(+) channels contributes to rhythmic firing of action potentials in mouse pancreatic beta cells. (1999)
- The voltage sensitive Lc-type Ca2+ channel is functionally coupled to the exocytotic machinery. (1999)
- Different interactions of cardiac and skeletal muscle ryanodine receptors with FK-506 binding protein isoforms (1997)
- Heterogeneity of Ca2+ gating of skeletal muscle and cardiac ryanodine receptors. (1997)
- Adrenaline stimulates glucagon secretion in pancreatic A-cells by increasing the Ca2+ current and the number of granules close to the L-type Ca2+ channels. (1997)
- Multisite regulation of insulin secretion by cAMP-increasing agonists (1997)
- Selective binding of FKBP12.6 by the cardiac ryanodine receptor. (1996)
- Fusion pore regulation by transient local generation of PI(4,5)P2 in pancreatic in β-cells
- Paracrine control of α-cell glucagon exocytosis is compromised in human type-2 diabetes
