Sebastian Barg
Professor vid Institutionen för medicinsk cellbiologi; Forskargrupp Sebastian Barg
- Telefon:
- 018-471 46 60
- E-post:
- sebastian.barg@mcb.uu.se
- Besöksadress:
- BMC
Husargatan 3
751 23 Uppsala - Postadress:
- Box 571
751 23 UPPSALA
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Kort presentation
Denna text finns inte på svenska, därför visas den engelska versionen.
My research group studies the cell biology of insulin secretion using advanced light microscopy (TIRF, single molecule imaging) and patch clamp electrophysiology; a particular focus is on the regulated exocytosis of secretory granules. I teach cell biology and general endocrinology.
Biografi
Denna text finns inte på svenska, därför visas den engelska versionen.
- 1995 MSc Biochemistry Free University Berlin
- 1995 undergraduate thesis Vanderbilt University (with Sid Fleischer)
- 2001 PhD Medical Sciences Lund University (with P Rorsman)
- 2002 Assistant professor (Swedish Science Council/Vetenskapsrådet)
- 2002-06 postdoc Vollum Institute Portland OR (with W Almers)
- 2007 research assistant professor Oregon Health & Science University
- 2007 lecturer Imperial College London
- 2008 Assistant professor Uppsala University
- 2011 Associate professor Uppsala University
honors:
- 2001 best PhD thesis Medical faculty Lund
- 2002 Swedish Research Council (VR) assistant professor position
- 2002 EMBO longterm fellowship
- 2002 STINT fellowship award
- 2002 Swedish Society for Medical Research fellowship award
- 2002 Albert Renold Career Development Award (EFSD)
- 2005 Wallenberg fellowship award in Nano/micro Science
- 2008 Zetterling fellowship award
- 2008-11 Göran Gustafsson award in human biology
Publikationer
Urval av publikationer
- Paracrine control of α-cell glucagon exocytosis is compromised in human type-2 diabetes. (2020)
- Fusion pore regulation by cAMP/Epac2 controls cargo release during insulin exocytosis (2019)
- Glucose-Dependent Granule Docking Limits Insulin Secretion and Is Decreased in Human Type 2 Diabetes (2018)
- Syntaxin clusters at secretory granules in a munc18-bound conformation (2018)
- Ca2+ channel clustering with insulin-containing granules is disturbed in type 2 diabetes (2017)
- Contact-induced clustering of syntaxin and munc18 docks secretory granules at the exocytosis site (2014)
- Syntaxin clusters assemble reversibly at sites of secretory granules in live cells (2010)
- Delay between fusion pore opening and peptide release from large dense-core vesicles in neuroendocrine cells (2002)
Senaste publikationer
- SEC14-like condensate phase transitions at plasma membranes regulate root growth in Arabidopsis (2023)
- Local PI(4,5)P2 signaling inhibits fusion pore expansion during exocytosis (2023)
- The β-cell primary cilium is an autonomous Ca2+ compartment for paracrine GABA signaling (2023)
- Restoration of PITPNA in Type 2 diabetic human islets reverses pancreatic beta-cell dysfunction (2023)
- Functional, metabolic and transcriptional maturation of human pancreatic islets derived from stem cells. (2022)
Alla publikationer
Artiklar
- SEC14-like condensate phase transitions at plasma membranes regulate root growth in Arabidopsis (2023)
- Local PI(4,5)P2 signaling inhibits fusion pore expansion during exocytosis (2023)
- The β-cell primary cilium is an autonomous Ca2+ compartment for paracrine GABA signaling (2023)
- Restoration of PITPNA in Type 2 diabetic human islets reverses pancreatic beta-cell dysfunction (2023)
- Functional, metabolic and transcriptional maturation of human pancreatic islets derived from stem cells. (2022)
- Alternative splicing encodes functional intracellular CD59 isoforms that mediate insulin secretion and are down-regulated in diabetic islets (2022)
- Paracrine control of α-cell glucagon exocytosis is compromised in human type-2 diabetes. (2020)
- In pancreatic islets from type 2 diabetes patients, the dampened circadian oscillators lead to reduced insulin and glucagon exocytosis (2020)
- Fusion pore regulation by cAMP/Epac2 controls cargo release during insulin exocytosis (2019)
- The Endoplasmic Reticulum Chaperone Glucose-Regulated Protein 94 is Essential for Proinsulin Handling (2019)
- Glucose-Dependent Granule Docking Limits Insulin Secretion and Is Decreased in Human Type 2 Diabetes (2018)
- Functional Characterization of Native, High-Affinity GABAA Receptors in Human Pancreatic β Cells (2018)
- PtdIns(4,5)P2 is not required for secretory granule docking (2018)
- Syntaxin clusters at secretory granules in a munc18-bound conformation (2018)
- Recruitment of Epac2A to Insulin Granule Docking Sites Regulates Priming for Exocytosis (2017)
- Ca2+ channel clustering with insulin-containing granules is disturbed in type 2 diabetes (2017)
- Plasma membrane PI(4,5)P-2 is critical for secretory granule exocytosis (2017)
- MiR-335 overexpression impairs insulin secretion through defective priming of insulin vesicles (2017)
- How Kiss-and-Run Can Make Us Sick (2016)
- Statistical Frailty Modeling for Quantitative Analysis of Exocytotic Events Recorded by Live Cell Imaging (2016)
- Plasma Membrane Phosphatidylinositol 4,5-Bisphosphate Regulates Ca2+-Influx and Insulin Secretion from Pancreatic beta Cells (2016)
- Quantitative Imaging of the Exocytosis Machinery Assembly (2015)
- Survey of Red Fluorescence Proteins as Markers for Secretory Granule Exocytosis (2015)
- G-protein coupled receptor dynamics in insulin secreting cells (2015)
- MiR-335 regulates exocytotic proteins and affects glucose-stimulated insulin secretion through decreased Ca2+-dependent exocytosis in beta cells (2015)
- Contact-induced clustering of syntaxin and munc18 docks secretory granules at the exocytosis site (2014)
- The Complement Inhibitor CD59 Regulates Insulin Secretion by Modulating Exocytotic Events (2014)
- CD59 is required for pancreatic beta cell insulin exocytosis (2014)
- In Intact Islets Interstitial GABA Activates GABA(A) Receptors That Generate Tonic Currents in alpha-Cells (2013)
- Aberrant association between vascular endothelial growth factor receptor-2 and VE-cadherin in response to vascular endothelial growth factor-a in Shb-deficient lung endothelial cells (2013)
- Multivesicular exocytosis in rat pancreatic beta cells (2012)
- PPARβ/δ affects pancreatic β cell mass and insulin secretion in mice (2012)
- Quantitative analysis of t-SNARE and Ca2+-channel clusters near secretory granules (2010)
- Syntaxin clusters assemble reversibly at sites of secretory granules in live cells (2010)
- cAMP Mediators of Pulsatile Insulin Secretion from Glucose-stimulated Single β-Cells (2010)
- Single secretory granules of live cells recruit syntaxin-1 and synaptosomal associated protein 25 (SNAP-25) in large copy numbers (2010)
- Defective secretion of islet hormones in chromogranin-B deficient mice (2010)
- TCF7L2 regulates late events in insulin secretion from pancreatic islet beta-cells (2009)
- High extracellular glucose inhibits exocytosis through disruption of syntaxin 1A-containing lipid rafts (2009)
- Impaired glucose homeostasis in Shb-/- mice (2009)
- Granule docking and cargo release in pancreatic β-cells (2008)
- Compensatory endocytosis in chromaffin cells (2008)
- Release of the styryl dyes from single synaptic vesicles in hippocampal neurons. (2008)
- Membrane phosphoinositides control insulin secretion through their effects on ATP-sensitive K+ channel activity. (2005)
- Selective nucleotide-release from dense-core granules in insulin-secreting cells (2005)
- Insulin secretion (2004)
- Large dense-core vesicle exocytosis in pancreatic beta-cells monitored by capacitance measurements. (2004)
- Mechanisms of exocytosis in insulin-secreting B-cells and glucagon-secreting A-cells. (2003)
- SUR1 regulates PKA-independent cAMP-induced granule priming in mouse pancreatic B-cells. (2003)
- A subset of 50 secretory granules in close contact with L-type Ca2+ channels accounts for first-phase insulin secretion in mouse beta-cells. (2002)
- Delay between fusion pore opening and peptide release from large dense-core vesicles in neuroendocrine cells (2002)
- Characterization of a naturally occurring mutation (L107I) in the HNF1 alpha (MODY3) gene. (2002)
- Differential activation by Ca2+, ATP and caffeine of cardiac and skeletal muscle ryanodine receptors after block by Mg2+. (2002)
- Effect of mutant alpha-synuclein on dopamine homeostasis in a new human mesencephalic cell line. (2002)
- Fast insulin secretion reflects exocytosis of docked granules in mouse pancreatic B-cells. (2002)
- Sulfonylurea-mediated stimulation of insulin exocytosis via an ATP-sensitive K+ channel-independent action. (2002)
- Priming of insulin granules for exocytosis by granular Cl(-) uptake and acidification (2001)
- Fast exocytosis with few Ca(2+) channels in insulin-secreting mouse pancreatic B cells (2001)
- Tight coupling between electrical activity and exocytosis in mouse glucagon-secreting alpha-cells. (2000)
- Patch-clamp characterisation of somatostatin-secreting -cells in intact mouse pancreatic islets. (2000)
- Regulation of glucagon release in mouse -cells by KATP channels and inactivation of TTX-sensitive Na+ channels. (2000)
- Tolbutamide stimulates exocytosis of glucagon by inhibition of a mitochondrial-like ATP-sensitive K+ (KATP) conductance in rat pancreatic A-cells. (2000)
- The Cell Physiology of Biphasic Insulin Secretion. (2000)
- The stimulatory action of tolbutamide on Ca2+-dependent exocytosis in pancreatic beta cells is mediated by a 65-kDa mdr-like P-glycoprotein (1999)
- Voltage-gated and resting membrane currents recorded from B-cells in intact mouse pancreatic islets. (1999)
- Activation of Ca(2+)-dependent K(+) channels contributes to rhythmic firing of action potentials in mouse pancreatic beta cells. (1999)
- The voltage sensitive Lc-type Ca2+ channel is functionally coupled to the exocytotic machinery. (1999)
- Different interactions of cardiac and skeletal muscle ryanodine receptors with FK-506 binding protein isoforms (1997)
- Heterogeneity of Ca2+ gating of skeletal muscle and cardiac ryanodine receptors. (1997)
- Adrenaline stimulates glucagon secretion in pancreatic A-cells by increasing the Ca2+ current and the number of granules close to the L-type Ca2+ channels. (1997)
- Multisite regulation of insulin secretion by cAMP-increasing agonists (1997)
- Selective binding of FKBP12.6 by the cardiac ryanodine receptor. (1996)
- Fusion pore regulation by transient local generation of PI(4,5)P2 in pancreatic in β-cells
- Paracrine control of α-cell glucagon exocytosis is compromised in human type-2 diabetes
