Joakim Näsvall
Forskare vid Institutionen för medicinsk biokemi och mikrobiologi; Infektioner och immunitet; Joakim Näsvall
- Mobiltelefon:
- 070-697 22 36
- E-post:
- joakim.nasvall@imbim.uu.se
- Besöksadress:
- BMC
Husargatan 3
752 37 UPPSALA - Postadress:
- Box 582
751 23 UPPSALA
- ORCID:
- 0000-0002-6831-3105
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Forskning
Evolution av nya gener
Vår forskning fokuserar på en väldigt grundläggande fråga i evolutionsbiologi; hur nya gener bildas och hur de evolverar. En ny gen kan uppkomma i ett genom på tre olika sätt:
(A) Horisontell (lateral) genöverföring, då en gen förs over från en annan organism. (B) De novo-evolution, då en funktion uppkommer i en tidigare icke-funktionell DNA-sekvens. (C) Duplikation-divergens, då en ny funktion evolverar i en kopia av en duplicerad gen. Vår nuvarande forskning berör i huvudsak duplikation-divergens.
Vi använder experimentell evolution och genetiska metoder i bakteriella modellsystem för att studera hur genduplikation och –amplifiering, neutrala mutationer, “trade-offs” mellan olika funktioner och andra faktorer påverkar uppkomsten av nya funktioner och deras vidare evolution. Vi är också intresserade av andra frågor, som evolution av komponenterna i translationsapparaten och den genetiska koden.
Publikationer
Urval av publikationer
- Collateral toxicity limits the evolution of bacterial Release Factor 2 towards total omnipotence (2020)
- Synonymous mutations in rpsT lead to ribosomal assembly defects that can be compensated by mutations in fis and rpoA (2020)
- Genetic adaptation to growth under laboratory conditions in Escherichia coli and Salmonella enterica (2018)
- Experimental Determination and Prediction of the Fitness Effects of Random Point Mutations in the Biosynthetic Enzyme HisA (2018)
- Direct and Inverted Repeat stimulated excision (DIRex) (2017)
- Duplication-Insertion Recombineering (2017)
- Evolution of New Functions De Novo and from Preexisting Genes (2015)
- Real-Time Evolution of New Genes by Innovation, Amplification, and Divergence (2012)
- Activation of cryptic aminoglycoside resistance in Salmonella enterica (2011)
Senaste publikationer
- Collateral toxicity limits the evolution of bacterial Release Factor 2 towards total omnipotence (2020)
- Synonymous mutations in rpsT lead to ribosomal assembly defects that can be compensated by mutations in fis and rpoA (2020)
- Mutational Pathways and Trade-Offs Between HisA and TrpF Functions (2020)
- Selection for novel metabolic capabilities in Salmonella enterica (2019)
- Genetic adaptation to growth under laboratory conditions in Escherichia coli and Salmonella enterica (2018)
Alla publikationer
Artiklar
- Collateral toxicity limits the evolution of bacterial Release Factor 2 towards total omnipotence (2020)
- Synonymous mutations in rpsT lead to ribosomal assembly defects that can be compensated by mutations in fis and rpoA (2020)
- Mutational Pathways and Trade-Offs Between HisA and TrpF Functions (2020)
- Selection for novel metabolic capabilities in Salmonella enterica (2019)
- Genetic adaptation to growth under laboratory conditions in Escherichia coli and Salmonella enterica (2018)
- Experimental Determination and Prediction of the Fitness Effects of Random Point Mutations in the Biosynthetic Enzyme HisA (2018)
- Structural mechanism of AadA, a dual specificity aminoglycoside adenylyltransferase from Salmonella enterica (2018)
- Evolution of antibiotic resistance without antibiotic exposure (2017)
- Structural and functional innovations in the real-time evolution of new (βα)8 barrel enzymes (2017)
- Direct and Inverted Repeat stimulated excision (DIRex) (2017)
- Duplication-Insertion Recombineering (2017)
- Compensating the fitness costs of synonymous mutations (2016)
- Evolution of New Functions De Novo and from Preexisting Genes (2015)
- Structure of AadA from Salmonella enterica (2015)
- Two-step Ligand Binding in a (βα)8 Barrel Enzyme (2015)
- Minor Fitness Costs in an Experimental Model of Horizontal Gene Transfer in Bacteria (2014)
- Real-Time Evolution of New Genes by Innovation, Amplification, and Divergence (2012)
- Activation of cryptic aminoglycoside resistance in Salmonella enterica (2011)
- Intermittent selection directs evolution of a specialist enzyme to become a generalist
- Evolvability of orthologous genes
- Crystal structure of AadA at 2.5 Å resolution - an aminoglycoside 3" adenyltransferase
- Synonymous mutations that reduce S20 levels can be compensated by mutations in fis and rpoA
- Genetic adaptations to growth under laboratory conditions in Escherichia coli and Salmonella enterica
- Functional trade-offs during evolution of new functions in the HisA-TrpF system
- Ribosomal protein L1 is required for balanced formation of ribosomal subunits
- Experimental evolution of novel metabolic capabilities in Salmonella enterica
